<p>I. Human Sperm Chromatin: Structure and Function</p><p>1. Sperm Chromatin: An Overview <br>Rodney Balhorn</p><p>2. Spermatogenesis: An Overview <br>Rakesh Sharma and Ashok Agarwal</p><p>3. Sperm Nucleoproteins<br>Rafael Oliva and Judit Castillo</p><p>4. The Relationship Between Chromatin Structure and DNA Damage in Mammalian Spermatozoa<br>Kenneth Dominguez, Chris D. R. Arca, and W. Steven Ward</p><p>5. Chromosome Positioning in Spermatozoa<br>Andrei Zalensky, Olga Mudrak, and Irina Zalenskaya </p><p>6. Sperm Mitochondrial DNA<br>Justin C. St. John and Bianca St. John</p><p>7. The Sperm Epigenome<br>Donovan Chan and Jacquetta Trasler </p><p>8. RNA Expression in Germ Cells During Spermatogenesis (Germ Cell Transcriptome) <br>Tin-Lap Lee, Albert Hoi-Hung Cheung, Owen M. Rennert, and Wai-Yee Chan</p><p>II. Laboratory Evaluation of Sperm Chromatin</p><p>9. Sperm Chromatin Structure Assay (SCSA®): 30 Years’ Experience with the SCSA®<br>Donald P. Evenson </p><p>10. Sperm Chromatin Dispersion Test: Technical Aspects and Clinical Applications<br>Jaime Gosálvez, Carmen López-Fernández, and José Luís Fernández </p><p>11. Basic and Clinical Aspects of the Sperm Chromomycin A3 Assay <br>Giancarlo Manicardi, Davide Bizzaro, and Denny Sakkas</p><p>12. Cytochemical Tests for Sperm Chromatin Maturity<br>Igor Tsarev and Juris Erenpreiss </p><p>13. Acridine Orange Test for Assessment of Human Sperm DNA Integrity<br>Alex C. Varghese, C. Fischer–Hammadeh, and M. E. Hammadeh </p><p>14. Laboratory Evaluation of Sperm Chromatin: TUNEL Assay<br>Rakesh Sharma and Ashok Agarwal</p><p>15. The Basic and Clinical Aspects of the Sperm Comet Assay<br>Luke Simon and Sheena E. M. Lewis</p><p>16. Assays Used in the Study of Sperm Nuclear Proteins<br>Timothy G. Jenkins, Benjamin R. Emery, and Douglas T. Carrell</p><p>17. Sperm Epigenetic Profile<br>Cristina Joana Marques, Alberto Barros, and Mário Sousa</p><p>18. Clinical Significance of Sperm RNA<br>Jean-Pierre Dadoune, Isabelle Galeraud-Denis, and Serge Carreau </p><p>III. Biological and Clinical Determinants of Sperm Chromatin Damage</p><p>19. Role of Oxidative Stress in the Etiology of Sperm DNA Damage<br>R. John Aitken and Geoffry N. De Iuliis</p><p>20. Abortive Apoptosis and Sperm Chromatin Damage<br>Hasan M. El-Fakahany and Denny Sakkas</p><p>21. Spermiogenesis in Sperm Genetic Integrity<br>Marie-Chantal Grégoire, Frédéric Leduc, and Guylain Boissonneault</p><p>22. Male Subfertility and Sperm Chromatin Damage<br>Mona Bungum, Aleksander Giwercman, and Marcello Spanò </p><p>23. Aging and Sperm DNA Damage<br>Fábio F. Pasqualotto and Eleonora B. Pasqualotto</p><p>24. Cancer in Males: Implications for Sperm Quality, Fertility, and Progeny Outcome<br>Peter Chan and Bernard Robaire</p><p>25. Sperm Chromatin and Environmental Factors<br>Aleksander Giwercman</p><p>26. Effects of Male Accessory Gland Infection on Sperm Parameters<br>Aldo E. Calogero, Sandro La Vignera, Rosita A. Condorelli, Rosario D’Agata, and Enzo Vicari</p><p>IV. Sperm Chromatin and Assisted Reproductive Technology Outcomes</p><p>27. The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity<br>Dan Yu, Luke Simon, and Sheena E. M. Lewis</p><p>28. Structure of Chromatin in Human Sperm Bound to Hyaluronic Acid: The Benefits of PICSI Dish Mediated Sperm Selection<br>Leyla Sati and Gabor Huszar</p><p>29. Electrophoretic Sperm Separation<br>Steven Fleming and R. John Aitken</p><p>30. Antioxidants and Sperm DNA Damage<br>Armand Zini and Maria San Gabriel</p><p>31. Sperm Chromatin and ART (IUI, IVF, and ICSI) Pregnancy<br>Mona Bungum</p><p>32. Sperm DNA Damage and Pregnancy Loss After IVF/ICSI<br>Armand Zini and Jason Matthew Boman</p><p>33. Postnatal Effects of Sperm Chromatin Damage<br>Miriam Pérez-Crespo, Raúl Fernández-González, Miguel Ángel Ramírez, Eva Pericuesta, Alexandra Calle, and Alfonso Gutiérrez-Adán</p><p>34. Evaluation of Chromatin and DNA Integrity in Testicular Sperm <br>Armand Zini and Naif Al-Hathal</p><p>V. Protocols and Integrity Tests</p><p>35. The Alkaline Comet Assay<br>Sheena E. M. Lewis </p><p>36. Sperm Chromatin Structure Assay (SCSA)<br>Donald P. Evenson </p><p>37. Measurement of DNA Damage in Spermatozoa by TUNEL Assay<br>Rakesh Sharma and Ashok Agarwal</p><p>38. Clinical Utility of Sperm DNA Integrity Tests<br>Armand Zini</p>