B Cell Protocols

Preface v Contributors ix Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen 1(24) Richard R. Hardy Susan A. Shinton Characterization of B-Cell Maturation in the Peripheral Immune System 25(12) Rita Carsetti Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods 37(22) James W. Tung David R. Parks Wayne A. Moore Leonard A. Herzenberg Leonore A. Herzenberg Analysis of B-Cell Life-Span and Homeostasis 59(8) Irmgard Forster Differentiation of B Lymphocytes From Hematopoietic Stem Cells 67(10) Paulo Vieira Ana Cumano Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System 77(14) Faith M. Young Carl A. Pinkert Andrea Bottaro Conditional Gene Mutagenesis in B-Lineage Cells 91(20) Stefano Casola Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells 111(16) Kai-Michael Toellner Mahmood Khan Daniel Man-Yuen Sze T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences 127(12) Claudia Berek Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors 139(10) Kuo-I Lin Kathryn Calame Immunoglobulin Class Switching: In Vitro Induction and Analysis 149(12) Sven Kracker Andreas Radbruch In Vitro and In Vivo Assays of B-Lymphocyte Migration 161(12) Chantal Moratz John H. Kehrl Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo 173(16) Louise J. McHeyzer-Williams Michael G. McHeyzer-Williams B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization 189(24) Tilman Brummer Winfried Elis Michael Reth Michael Huber Isolation of Lipid Rafts From B Lymphocytes 213(12) Anu Cherukuri Shiang-Jong Tzeng Arun Gidwani Hae Won Sohn Pavel Tolar Michelle D. Snyder Susan K. Pierce Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells 225(14) Ralf Kuppers Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice 239(22) Helen Ferry Richard J. Cornall Analysis of B-Cell Signaling Using DT40 B-Cell Line 261(10) Tomoharu Yasuda Tadashi Yamamoto B-Cell Development and Pre-B-1 Cell Plasticity In Vitro 271(12) Antonius G. Rolink Index 283