Bioconjugation Protocols : Strategies and Methods

Preface v Contributors ix PART I ANTIBODY AND ENZYME CONJUGATES 1 Streptavidin-Biotin Crosslinking of Therapeutic Enzymes With Carrier Antibodies: Nanoconjugates for Protection Against Endothelial Oxidative Stress Vladimir V. Shuvaev, Thomas Dziubla, Rainer Wiewrodt, and Vladimir R. Muzykantov 3(18) 2 Characterization of Endothelial Internalization and Targeting of Antibody-Enzyme Conjugates in Cell Cultures and in Laboratory Animals Silvia Muro, Vladimir R. Muzykantov, and Juan-Carlos Murciano 21(16) 3 Smart Polymer-Streptavidin Conjugates Patrick S. Stayton, Zhongli Ding, and Allan S. Hoffman 37(8) 4 Conjugates of Peptides and Proteins to Polyethylene Glycols Margherita Morpurgo and Francesco M. Veronese 45(26) 5 Chemical Production of Bispecific Antibodies Robert F. Graziano and Paul Guptill 71(16) 6 Preparation of Immunoconjugates Using Antibody Oligosaccharide Moieties Carl-Wilhelm Vogel 87(22) 7 Synthesis of Hapten-Protein Conjugates Using Microbial Transglutaminase Markus Meusel 109(18) PART II NUCLEIC ACID CONJUGATES 8 Fluorescent Sample Labeling for DNA Microarray Analyses Verena Beier, Andrea Bauer, Michael Baum, and Jörg D. Hoheisel 127(10) 9 High-Density Labeling of DNA for Single Molecule Sequencing Susanne Brakmann 137(8) 10 Sequence-Specific DNA Labeling Using Methyltransferases Goran Pljevaljcic, Falk Schmidt, Alexander Peschlow, and Elmar Weinhold 145(18) 11 Hapten Labeling of Nucleic Acids for Immuno-Polymerase Chain Reaction Applications Michael Adler 163(18) 12 Covalent Coupling of DNA Oligonucleotides and Streptavidin Florian Kukolka, Marina Lovrinovic, Ron Wacker, and Christof M. Niemeyer 181(16) 13 Synthesis of Oligonucleotide-Peptide and Oligonucleotide-Protein Conjugates David R. Corey 197(10) 14 Synthesis of Peptide Nucleic Acid-Peptide Conjugates Kunihiro Kaihatsu and David R. Corey 207(10) PART III GLYCOSYL AND LIPID CONJUGATES 15 Protein Lipidation Jürgen Kuhlmann 217(4) 16 Synthesis of Lipidated Peptides Ines Heinemann, Martin Völkert, and Herbert Waldmann 221(12) 17 In Vitro Semisynthesis and Applications of C-Terminally Modified Rab Proteins Thomas Durek, Roger S. Goody, and Kirill Alexandrov 233(12) 18 Generation and Characterization of Ras Lipoproteins Based on Chemical Coupling Melanie Wagner and Jürgen Kuhlmann 245(10) 19 Conjugation of Glycopeptide Thioesters to Expressed Protein Fragments: Semisynthesis of Glycosylated Interleukin-2 Thomas J. Tolbert and Chi-Huey Wong 255(12) 20 Subtilisin-Catalyzed Glycopeptide Condensation Thomas J. Tolbert and Chi-Huey Wong 267(16) PART IV BIOFUNCTIONALIZATION OF SURFACES 21 Peptide Nucleic Acid Microarrays Anette Jacob, Ole Brandt, Achim Stephan, and Jörg D. Hoheisel 283(12) 22 Synthesis and Characterization of Deoxyribonucleic Acid-Conjugated Gold Nanoparticles Pompi Hazarika, Tatiana Giorgi, Martina Reibner, Buelent Ceyhan, and Christof M. Niemeyer 295(10) 23 Biofunctionalization of Carbon Nanotubes for Atomic Force Microscopy Imaging Adam T. Woolley 305(16) Index 321