Developmental Hematopoiesis : Methods and Protocols

Preface v Contributors xi PART I: GENETIC APPROACHES 1 Gene-Targeting and Transgenic Strategies for the Analysis of Hematopoietic Development in the Mouse Hanna K.A. Mikkola and Stuart H. Orkin 3(20) 2 Inducible Transgene Expression in Mouse ES Cells David T. Ting, Michael Kyba, and George Q. Daley 23(24) 3 Quantitative Trait Analysis in the Investigation of Function and Aging in Hematopoietic Stem Cells Hans-Willem Snoeck 47(18) PART II: ANALYSIS OF CELL FATE OUTCOMES IN TRANSPLANTATION MODELS 4 Flow Cytometric Analysis of Hematopoietic Development Edward F. Srour and Mervin C. Yoder 65(16) 5 Reconstitution of Hematopoiesis Following Intrauterine Transplantation of Stem Cells Elisabeth H. Javazon, Aziz M. Merchant, Enrico Danzer, and Alan W. Flake 81(14) 6 Reconstitution of Hematopoiesis Following Transplantation Into Neonatal Mice Scott A. Johnson and Mervin C. Yoder 95(14) PART III: ANIMAL MODELS Drosophila 7 Hemocyte Development During Drosophila Embryogenesis Robert A. Schulz and Nancy Fossett 109(14) Xenopus 8 Tracking and Programming Early Hematopoietic Cells in Xenopus Embryos Maggie Walmsley, Aldo Ciau-Litz, and Roger Patient 123(14) 9 Fate Mapping Hematopoietic Lineages in the Xenopus Embryo Mary Constance Lane and Michael D. Sheets 137(12) 10 Analyses of Immune Responses to Ontogeny-Specific Antigens Using an Inbred Strain of Xenopus Iaevis (J Strain) Yumi Izutsu and Mitsugu Maeno 149(10) 11 Use of Flow Cytometry and Combined DNA/Surface Staining for Analysis of Hematopoietic Development in the Xenopus Embryo James B. Turpen 159(12) Zebra fish 12 Analysis of Hematopoietic Development in the Zebrafish Noelle N. Paffett-Lugassy and Leonard I. Zon 171(28) 13 Imaging Early Macrophage Differentiation, Migration, and Behaviors in Live Zebrafish Embryos Philippe Herbomel and Jean-Pierre Levraud 199(16) Chick 14 In Vivo Methods to Analyze Cell Origins, Migrations, Homing, and Interactions in the Blood, Vascular, and Immune Systems of the Avian and Mammalian Embryo Françoise Dieterlen-Lièvre, Sophie Creuzet, and Josselyne Salaün 215(16) Mouse 15 Mouse Embryonic Explant Culture System for Analysis of Hematopoietic and Vascular Development Margaret H. Baron and Deanna Mohn 231(26) 16 Hematopoietic Stem Cell Enrichment From the AGM Region of the Mouse Embryo Catherine Robin and Elaine Dzierzak 257(16) 17 Hematopoietic Stem Cell Development During Mouse Embryogenesis Julien Y. Bertrand, Sebastien Giroux, Ana Cumano, and Isabelle Godin 273(16) 18 Analysis of Hematopoietic Progenitors in the Mouse Embryo James Palis and Anne Koniski 289(14) 19 In Vivo and In Vitro Assays of Thymic Organogenesis Julie Gordon, Valerie A. Wilson, Billie A. Moore-Scott, Nancy R. Manley, and C. Clare Blackburn 303(8) 20 Retroviral Transduction in Fetal Thymic Organ Culture Bronwyn M. Owens, Robert G. Hawley, and Lisa M. Spain 311(12) 21 Expansion and Differentiation of Immature Mouse and Human Hematopoietic Progenitors Helmut Dolznig, Andrea Kolbus, Cornelia Leberbauer, Uwe Schmidt, Eva-Maria Deiner, Ernst W. Müllner, and Hartmut Beug 323(22) 22 A New Clonal Assay System for Lymphoid and Myeloid Lineages Hiroshi Kawamoto and Yoshimoto Katsura 345(14) 23 Hematopoietic and Endothelial Development of Mouse Embryonic Stem Cells in Culture Kyunghee Choi, Yun Shin Chung, and Weh Jie Zhang 359(10) 24 Analysis of the Vascular Potential of Hematopoietic Stem Cells Steven M. Guthrie, Sergio Caballero, Robert N. Mames, Maria B. Grant, and Edward W. Scott 369(12) 25 Dynamic In Vivo Imaging of Mammalian Hematovascular Development Using Whole Embryo Culture Elizabeth A.V. Jones, Margaret H. Baron, Scott E. Fraser, and Mary E. Dickinson 381(14) 26 Fluorescent Protein-Cell Labeling and Its Application in Time-Lapse Analysis of Hematopoietic Differentiation Matthias Stadtfeld, Florencio Varas, and Thomas Graf 395(18) Human 27 Analysis of Hematopoietic Development During Human Embryonic Ontogenesis Manuela Tavian and Bruno Péault 413(12) 28 Hematopoietic Development of Human Embryonic Stem Cells in Culture Xinghui Tian and Dan S. Kaufman 425(14) PART IV: BIOINFORMATICS AND FUNCTIONAL GENOMICS 29 A Functional Genomics Approach to Hematopoietic Stem Cell Regulation Jason A. Hackney and Kateri A. Moore 439(14) Index 453