Microbiology Laboratory Exercises

Part 1 Observing the Microbial World The Compound Microscope Microscopic Measurement Aseptic Transfer Techniques Preparing a Wet Mount and Observing Microorganisms Algae Fungi, Protozoa, and Microscopic Animalia Part 2 Bacterial Staining TechniquesPreparing a Smear and Simple Stain Gram Staining Acid-Fast Staining Endospore Staining Part 3 Growing Microorganisms Ubiquity and Culture CharacteristicsPour-Plate Techniques Streak-Plate and Spread-Plate TechniquesHand Washing Quantifying Bacteria Anaerobic Culture: Oxygen Requirements Bacteriophage Part 4 Influencing and Controlling Microbial Growth Temperature and pH Disinfectants and Antiseptics Antibiotic Disk Sensitivity Testing Part 5 Microbial Metabolic Reactions Selected Microbial Enzymes: Amylase, Lipase, Catalase, Cytochrome Oxidase, Cysteine Desulfhydrase Enzymatic Attacks on Nitrogen-Containing Substrates: Urea Hydrolysis, Nitrate Reduction, Proteolysis of Gelatin Carbohydrate FermentationThe IMViC Tests: A Set of Biochemical Reactions Selective and Differential Media Multiple-Test Media and Systems Part 6 Medical MicrobiologyMedically Significant Gram-Positive Cocci Intestinal Pathogens: Campylobacter and Selected Enterobacteriaceae Mouth, Throat, and Lower Respiratory System Flora Urinary Tract Normal Flora Part 7 Identifying Bacterial Unknowns Bacterial Unknowns: Isolation, Stock Culture, and Morphology Identifying Bacterial Unknowns Part 8 Genetics and Immunology SerologyGenetic Engineering and Bacterial Transformation