Section I. Nitric Oxide-Metabolising and Detoxifying Enzymes<br><br>Chapter 1<br>Structural Studies on Flavodiiron Proteins <br>João B. Vicente, Maria Arménia Carrondo, Miguel Teixeira and Carlos Frazão<br><br>Chapter 2<br>Biochemical, Spectroscopic and Thermodynamic Properties of Flavodiiron Proteins<br>João B. Vicente, Marta C. Justino, Vera L. Gonçalves, Lígia M. Saraiva and Miguel Teixeira<br><br>Chapter 3<br>Kinetic Characterization of the Escherichia coli Nitric Oxide Reductase Flavorubredoxin <br>João B. Vicente, Francesca M. Scandurra, Elena Forte, Maurizio Brunori, Paolo Sarti, Miguel Teixeira and Alessandro Giuffrè<br><br>Chapter 4<br>Escherichia coli cytochrome c-nitrite reductase NrfA<br>Thomas A. Clarke, Paul C. Mills, Susie R. Poock, Julea N. Butt1, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher Söderberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson<br><br>Chapter 5<br>The respiratory NO reductase (NorBC) from Paracoccus denitrificans<br>Sarah J. Field, Faye H. Thorndycroft, Andrey D. Matorin, <br>David J. Richardson and Nicholas J. Watmough<br><br>Chapter 6<br>Redox-controlled dinitrosyl formation at the diiron-oxo center of NorA<br>Rainer Cramm, Katja Strube<br><br>Chapter 7<br>Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor<br>Li Zhangand Hirofumi Shoun <br><br>Chapter 8 <br>A Quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain <br>Maria G. Mason, Rebecca S. Holladay, Peter Nicholls, Mark Shepherd and Chris E. Cooper<br><br>Section II. Sensor Proteins<br>Chapter 9<br>Cloning, Expression, and Purification of the N-terminal Heme Binding Domain of the Globin-Coupled Sensors<br>Jennifer A. Saito, Tracey Allen K. Freitas, and Maqsudul Alam<br><br>Chapter 10<br>Oxygen-Sensing Histidine-Protein Kinases: Assays of Ligand-Binding and Turnover of Response-Regulator Substrates<br>Marie-Alda Gilles-Gonzalez, Gonzalo Gonzalez, Eduardo H. S. Sousa, and Jason Tuckerman<br><br>Chapter 11<br>Reactions of nitric oxide and oxygen with FNR (regulator of Fumarate and Nitrate Reduction), a global transcriptional regulator, during anaerobic growth of Escherichia coli<br>Jason C. Crack, Nick E. Le Brun, Andrew J. Thomson, Jeffrey Green and Adrian J. Jervis<br><br>Chapter 12<br>Genome wide identification of binding sites for the nitric oxide sensitive transcriptional regulator NsrR. <br>Sam Efromovich, David Grainger, Diane Bodenmiller, and Stephen Spiro.<br><br>Chapter 13<br>Methods in Enzymology: Globins and other NO-reactive proteins. Characterization of the NO-reactive transcriptional activator NorR.<br>Benoît D’Autréaux, Nick Tucker, Stephen Spiro and Ray Dixon<br><br>Section III. Advanced Spectroscopic Methods<br><br>Chapter 14<br>Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni: A Comparative Study with Resonance Raman Spectroscopy <br>Changyuan Lu, Tsuyoshi Egawa, Masahiro Mukai, Robert K. Pooleand Syun-Ru Yeh<br><br>Chapter 15<br>The power of using continuous-wave and pulsed electron paramagnetic resonance methods for the structure analysis of the ferric forms and NO-ligated ferrous forms of globins.<br>Sabine Van Doorslaer and Filip Desmet<br><br>Chapter 16<br>Oxygen binding to haem proteins in solution, encapsulated in silica gels and in the crystalline state<br>Luca Ronda, Stefano Bruno, Serena Faggiano, Stefano Bettati and Andrea Mozzarelli<br><br>Chapter 17<br>Characterization of ligand migration mechanisms inside haemoglobins from the analysis of geminate rebinding kinetics<br>Stefania Abbruzzetti, Stefano Bruno, Serena Faggiano, Luca Ronda, Elena Grandi, Andrea Mozzarelli, Cristiano Viappiani<br><br>Chapter 18<br>Ligand Dynamics in Heme Proteins Observed by <br>Fourier Transform Infrared Spectroscopy at Cryogenic Temperatures<br>Karin Nienhaus, G. Ulrich Nienhaus<br><br>Chapter 19<br>Time-resolved x-ray crystallography of heme proteins<br>Vukica Šrajer and William E. Royer, Jr<br><br>Chapter 20<br>Structural Dynamics of Myoglobin<br>Maurizio Brunori, Dominque Bourgeois and Beatrice Vallone<br><br>Chapter 21<br>Use of the Conjugate Peak Refinement Algorithm for Identification of Ligand Binding Pathways in Globins<br>Stephen D. Golden and Kenneth W. Olsen<br><br>Chapter 22<br>Finding gas migration pathways in proteins using implicit ligand sampling<br>Jordi Cohen, Kenneth W Olsen, and Klaus Schulten<br><br>Chapter 23<br>Identification of Ligand Binding Pathways in Truncated Hemoglobins Using Locally Enhanced Sampling Molecular Dynamics<br>Stephen D. Golden and Kenneth W. Olsen