The Zebrafish: Genetics, Genomics and Informatics

<p>Part I: Forward and Reverse Genetics</p> <p>1. Gene Trap Mutagenesis for Generation of Conditional Mutations in Zebrafish – Wenbiao Chen</p> <p>2. Advances in Tol2 Transposon Mutagenesis – Koichi Kawakami</p> <p>3. OPEN Zinc Finger Nucleases – Keith Joung and Randy Peterson</p> <p>4. Retroviral-Mediated Insertional Mutagenesis in Zebrafish – Adam Amsterdam, Nancy Hopkins, and Shawn Burgess</p> <p>5. Genetic Screens for Maternal-Effects Mutations – Francisco Pelegri and Mary Mullins</p> <p>6. High-Throughput Target-Selected Gene Inactivation in Zebrafish – Derek Stemple</p> <p>7. Suppressor Screens in Haploids – Xiaoying Bai</p> <p>8. Transposons – Stephen Ekker</p> <p>9. Caged MOs – James Chen</p> <p>Part II: Transgenesis</p> <p>1. Advanced Transgenesis with Cre Recombinase – Christian Mosimann</p> <p>2. Transgenesis with phiC31 Recombinase – James Lister</p> <p>3. Nuclear Transfer – Jose Cibelli and Dr. Siripattarapravat</p> <p>Part III: The Zebrafish Genome and Mapping Technologies</p> <p>1. The Zebrafish Genome Project: Sequence Analysis and Annotation– Derek Stemple</p> <p>2. Molecular Cytogenetic Methodologies and a Bacterial Artificial Chromosome (BAC) Probe Panel Resource for Genomic Analyses in Zebrafish – Charles Lee and Amanda Smith</p> <p>3. Automated Analysis of Conserved Syntenies for the Zebrafish Genome – John Postlethwait, Julian Catchen</p> <p>4. The Zon Laboratory Guide to Positional Cloning in Zebrafish –Yi Zhou and Leonard Zon</p> <p>Part IV: Informatics and Comparative Genomics</p> <p>1. Data Warehouse ZFIN – Monte Westerfield and Judy Sprague</p> <p>2. Epigenetics: Methylation – Brad Cairns</p> <p>3. Chromatin Structure – Leonard Zon and Yi Zhou</p> <p>4. ChipSeq – Brad Cairns</p> <p>5. Micro RNAs – Graham Lieshke and Luke Pase</p> <p>6. Microarrays – Yi Zhou</p> <p>7. Sequence-Based Transcriptional Profiling – Shawn Burgess</p> <p>8. Epigenetic Regulation of Zebrafish Development – Elke Ober and co-author</p> <p>Part V: Infrastructure</p> <p>1. Zebrafish Facilities for Small and Large Laboratories – Christian Lawrence</p> <p>2. Genetic Backgrounds, Standard Lines, and their Husbandry – ZIRC, Zoltan</p>