Section I. General Resources <br>1. A survey of internet resources for mouse development <br>Thomas L. Saunders <br><br>Section II. Handling Mouse Lines <br>2. Transport of mouse lines by shipment of live embryos <br>Kevin A. Kelley <br>3. Strategies and considerations for distributing and recovering mouse lines <br>Yubin Du, Wen Xie, and Chengyu Liu <br>4. Archiving and distributing mouse lines by sperm cryopreservation, IVF, and embryo transfer <br>Hideko Takahashi and Chengyu Liu <br><br>Section III. Gametes and Embryos <br>5. Isolation and manipulation of mouse gametes and embryos <br>Eveline S. Litscher and Paul M. Wassarman <br>6. Cryopreservation of mouse gametes and embryos <br>Carlisle P. Landel <br>7. Ovarian follicle culture systems for mammals <br>David F. Albertini and Gokhan Akkoyunlu <br>8. Production of mouse chimaeras by aggregating pluripotent stem cells with embryos <br>Andras Nagy, Kristina Nagy, and Marina Gertsenstein <br>9. Production of cloned mice from somatic cells, ES cells, and frozen bodies <br>Sayaka Wakayama, Eiji Mizutani, and Teruhiko Wakayama <br>10. Nuclear transfer in mouse oocytes and embryos <br>Zhiming Han, Yong Cheng, Cheng-Guang Liang, and Keith E. Latham <br>11. Culture of whole mouse embryos at early post-implantation to organogenesis stages: developmental staging and methods <br>Jaime A. Rivera-Perez, Vanessa Jones, and Patrick P. L. Tam <br>12. In utero and ex utero surgery on rodent embryos <br>Valerie Ngo-Muller and Ken Mueoka <br><br>Section IV. Fertilization <br>13. Enhancement of IVF in the mouse by zona-drilling <br>Kevin A. Kelley <br>14. ICSI in the mouse <br>Paula Stein and Richard M. Schultz <br><br>Section V. ES and iPS Cells <br>15. A simple procedure for the efficient derivation of mouse ES cells <br>Esther Wong, Kenneth Ban, Rafidah Mutalif, Nancy A. Jenkins, Neal G. Copeland, and Colin L. Stewart <br>16. Producing fully ES cell-derived mice from 8-cell stage embryo injections <br>Thomas M. DeChiara, William T. Poueymirou, Wojtek Auerbach, David Frendewey, George D. Yancopoulos, and David M. Valenzuela <br>17. The loss-of-allele assay for ES cell screening and mouse genotyping <br>David Frendewey, Rostislav Chernomorsky, Lakeisha Esau, Jinsop Om, Yingzi Xue, Andrew J. Murphy, George D. Yancopoulos, and David M. Valenzuela <br>18. Induced pluripotent stem (iPS) cells <br>Holm Zaehres, Jeong Beom Kim, and Hans R. Schöler <br><br>Section VI. Imaging Mouse Development <br>19. Imaging mouse embryonic development <br>Ryan S. Udan and Mary E. Dickinson <br>20. Imaging mouse development with confocal time-lapse microscopy <br>Sonja Nowotschin, Anna Ferrer-Vaquer, and Anna-Katerina Hadjantonakis <br>21. Ultrasound and magnetic resonance microimaging of mouse development <br>Brian J. Nieman and Daniel H. Turnbull <br><br>Section VII. Hematopoiesis <br>22. Use of transgenic fluorescent reporter mouse lines to monitor hematopoietic and erythroid development during embryogenesis <br>Stuart T. Fraser, Joan Isern, and Margaret H. Baron <br>23. Identification and in vivo analysis of murine hematopoietic stem cells <br>Serine Avagyan, Yacine M. Amrani, and Hans-Willem Snoeck