Current Laboratory Techniques in Rabies Diagnosis, Research and Prevention, Volume 2

<p>I. Foreword<br>II. Introduction<br>III. Rabies Diagnosis<br>a) Demonstration of virus<br>i) Electron microscopy<br>ii) Virus isolation in animals, the mouse inoculation test (MIT)<br>iii) Virus isolation in cell culture, the rapid tissue culture inoculation test (RTCIT)<br>iv) Rat basophilic leukemia cell sensor<br>b) Demonstration of viral subunits and antigens<br>i) Purification of rabies viral subunits<br>ii) Preparation of fluorescent antibody conjugate in rabbits<br>iii) Preparation of fluorescent antibody conjugate in goats<br>iv) DFA (postmortem)<br>v) Skin biopsy<br>vi) Immuno histochemistry<br>c) Demonstration of viral nucleic acids<br>i) Gel based PCR<br>ii) In situ PCR<br>d) Demonstration of viral antibodies<br>1. Binding Assays<br>i) ELISA - Traditional<br>ii) IFA<br>iii) Counter Immunoelectrophoresis<br>2. Functional Assays<br>i) Mouse neutralization test<br>ii) RFFIT<br>iii) FAVN<br>e) Typing/differentiation of lyssaviruses<br>i) Antigenic typing by MAbs</p> <p>IV. Rabies Biologics<br>i) Rabies vaccines for humans or other animals<br>1. Purified chick embryo cell vaccine<br>2. Purified Vero Cell Rabies Vaccine<br>3. In vivo NIH potency testing of rabies vaccines<br>9. In vitro Potency testing of rabies vaccines<br>ii) Rabies antibodies/fragments<br>1. Production of polyclonal rabies immune globulin in humans<br>2. Production of polyclonal antibodies in horses<br>3. Production of polyclonal antibodies in birds<br>4. Production of full length human monoclonal antibodies using transgenic mice</p> <p>V. Development of Anti-Viral Approaches</p> <p>VI. Appendix<br>i. List of biologics recommended by WHO for human rabies prophylaxis</p>