The Zebrafish: Genetics, Genomics, and Transcriptomics

<p>Part 1. Forward and Reverse Genetics<br>1. Multiplex Conditional Mutagenesis in Zebrafish Using the CRISPR/Cas System<br>2. Tol2-Mediated Transgenesis, Gene Trapping and Enhancer Trapping, and the Gal4-UAS System<br>3. Genetic Screens for Mutations Affecting Adult Traits and Parental-Effect Genes<br>4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology<br>5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish<br>6. Precise Genome Editing by Homologous Recombination<br>7. The Zebrafish Genome Editing Toolkit<br>8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology<br>9. Tissue-Specific Gene Targeting Using CRISPR/Cas9</p> <p>Part 2. Transgenesis and Functional Genomics<br>10. Transcriptional Regulation Using the Q System in Transgenic Zebrafish<br>11. Contemporary Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments<br>12. Method  for Somatic Cell Nuclear Transfer in Zebrafish<br>13. Experimental Approaches to Studying the Nature and Impact of Splicing Variation in Zebrafish<br>14. PICCORO: A Technique for Manipulating the Activity of Transcription Factors with Blue Light</p> <p>Part 3. Transcriptomics<br>15. Tomo-seq: A Method to Obtain Genome-Wide Expression Data with Spatial Resolution<br>16. Cell-Type Specific Transcriptomic Analysis by Thiouracil Tagging in Zebrafish<br>17. RT-qPCR Gene Expression Analysis in Zebrafish: Preanalytical Precautions and Use of Expressed Repetitive Elements (ERE) for Normalization</p> <p>Part 4. Epigenetics<br>18. Genome-Wide DNA Methylation Profiling in Zebrafish<br>19. Histone Modifications in Zebrafish Development<br>20. Chromatin Immunoprecipitation and an Open Chromatin Assay in Zebrafish Erythrocytes<br>21. Assay for Transposase-Accessible Chromatin and Circularized Chromosome Conformation Capture, Two Methods to Explore the Regulatory Landscapes of Genes in Zebrafish<br>22. Epigenetic Regulation of Hematopoietic Stem Cell Development</p> <p>Part 5. Zebrafish Infrastructure<br>23. A Scientist’s Guide for Submitting Data to ZFIN<br>24. New Frontiers for Zebrafish Management<br>25. Aquaculture, Husbandry, and Shipping at the Zebrafish International Resource Center<br>26. Health Monitoring and Disease Prevention at the Zebrafish International Resource Center</p>