Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer

1. An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol<br>Emmanuel intégrative des tumeurs et immunothérapie Donnadieu, Irena Rajnpreht and Lene Vimeux<br>2. new protocol to measure mitochondria polarization/function at the immunological synapse<br>Manuel Izquierdo, Javier Ruiz Navarro and Victor Calvo<br>3. Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models<br>Teresa Lozano, Noa Beatriz Martín-Cófreces, Álvaro Gómez-Morón and Celia Martín-Otal<br>4. Investigation of the in vitro lytic capacity of CAR-T by microscopy'<br>Noa Beatriz Martín-Cófreces, Teresa Lozano, Álvaro Gómez-Morón and Celia Martín-Otal<br>5. Antigen-specific T cell cytotoxicity toward target tumor cells<br>Chong Sun and Helena Engel<br>6. In vitro expansion of stem-like memory T cells via D-mannose supplementation<br>Bo Huang, Guideng Li, Yajing Qiu and Cngcong Zhao<br>7. High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells.<br>Hélène Sophie DANIELS-TREFFANDIER, Loic Dupre, Beatriz CHAVES and Nicolas SOCQUET-JUGLARD<br>8. Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation<br>Yu Cheng, Rui Gao, Wanxin Huang and Yehe Kang<br>9. Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry<br>Massimo Vitale, Paola Orecchia, Guido Ferlazzo, Monica Parodi, Simonetta Astigiano, Valentina Delli Zotti, Filippo Piombo, Stefania Martini, Simona Pigozzi and Paolo Carrega<br>10. Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy.<br>Charles Daniel Murin, Emily Mace, Patrick Ross, Jessica Matthias, Daniel Leaman, Micahel Zwick and Scott Henderson<br>11. Assessment of NK cell cytotoxicity induced by il-15 based immunotherapy against cancer cells<br>Carole Seguin-Devaux, Camille Rolin, Gilles Iserentant and Aubin Pitiot<br>12. Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry<br>Sophie-Christin Linkenbach, Ayline Kübler and Tommaso Torcellan<br>13. Anti-tumor activity characterization of NK cell subsets by flow cytometry<br>Massimo Vitale, Carola Perrone, Paolo Canevali, Veronica Colamassaro, Elena Giusto, Silvia Tamburini, Gianluca Ubezio, Guido Ferlazzo and Raffaella Meazza<br>14. DNA Origami-Decorated Lipid Bilayer Systems for Interrogating the Spatial Requirements underlying B cell Immune-Synapse Formation<br>Bebhinn Treanor, Yuchen Hou, Chung -Yi Tseng, Raysa Orin, Emily Cohen and Leo Chou