Photosynthesis

Methods and Protocols

Specificaties
Gebonden, blz. | Engels
Springer New York | e druk, 2018
ISBN13: 9781493977857
Rubricering
Juridisch :
Springer New York e druk, 2018 9781493977857
Onderdeel van serie Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen

Samenvatting

This user-friendly book provides a range of classical and modern techniques for the study of photosynthesis in a manner accessible to a broad spectrum of researchers. Broken into four sections, it explores the measurement of physiological photosynthetic parameters, quantifying photosynthetic enzyme abundance and catalytic activity, visualizing cellular and sub-cellular phenotypes, and photosynthesis-inspired energy generation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and practical, Photosynthesis: Methods and Protocols aims to aid scientists working toward exciting developments in the understanding and application of photosynthesis.

Specificaties

ISBN13:9781493977857
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York

Inhoudsopgave

<p>Survey of Tools for Measuring In Vivo Photosynthesis.- Photosynthetic Gas Exchange in Land Plants at the Leaf Level.- Design and Use of a Digitally-Controlled Device for Accurate, Multiplexed Gas Exchange Measurements of the Complete Foliar Parts of Plants.- Measuring Canopy Gas Exchange Using CAnopy Photosynthesis and Transpiration Systems (CAPTS).- Light-Response Curves in Land Plants.- Chlorophyll Fluorescence on the Fast Timescale.- Sub-Saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield.- Chlorophyll Fluorescence Imaging.- Measurement of O<sub>2</sub> Uptake and Evolution in Leaves In Vivo Using Stable Isotopes and Membrane Inlet Mass Spectrometry.- Using Carbon Stable Isotopes to Study C<sub>3</sub> and C<sub>4</sub> Photosynthesis: Models and Calculations.- Liquid-Phase Measurements of Photosynthetic Oxygen Evolution.- Quantification of Photosynthetic Enzymes in Leaf Extracts by Immunoblotting.- Extraction of Rubisco to Determine Catalytic Constants.- Spectrophotometric Determination of Rubisco Activity and Activation State in Leaf Extracts.- Creating Leaf Cell Suspensions for Characterization of Mesophyll and Bundle Sheath Cellular Features.- Determining the Subcellular Localization of Fluorescently-Tagged Proteins Using Protoplasts Extracted from Transiently-Transformed Nicotiana benthamiana Leaves.- 3D Clearing and Molecular Labeling in Plant Tissues.- Evaluation of Lipids for the Study of Photosynthetic Membranes.- “Click” Methodology for the Functionalization of Water Oxidation Catalyst Iridium Oxide Nanoparticles with Hydrophobic Dyes for Artificial Photosynthetic Constructs.- Biophotovoltaics: Design and Study of Bio-Electrochemical Systems for Biotechnological Applications and Metabolic Investigation.</p>

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        Photosynthesis