Avian Influenza Virus

Avian Influenza Virus Table of Contents 1. A brief introduction to avian influenza virus Erica Spackman 2. Avian Influenza Virus Sample Types, Collection, and Handling Mary-Lea Killian 3. Avian influenza virus RNA extraction from tissue and swab material Erica Spackman and David L. Suarez 4. Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR Erica Spackman and David L. Suarez 5. Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR Erica Spackman and David L. Suarez 6. Avian influenza virus isolation and propagation in chicken eggs Peter R. Woolcock 7. Hemagglutination Assay for Avian Influenza Virus Mary-Lea Killian 8. Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus Janice C. Pedersen 9. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity Janice C. Pedersen 10. Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues Mary Pantin-Jackwood 11. Wild bird Surveillance for Avian Influenza Virus Justin D. Brown and David E. Stallknecht 12. Reverse Genetics of Avian Influenza Virus Chang-Won Lee and David L. Suarez 13. Evaluating the Cell Mediated Immune Response of Avian Species to Avian Influenza Viruses Darrell R. Kapczynski 14. Measurment of avian cytokines with real-time RT-PCR following infection with avian influenza virus Darrell R. Kapczynski and Michael H. Kogut