Chloroplast Biotechnology

<p>Part I:  Background</p><p>1. The Plastid Genomes of Flowering Plants</p><p>Tracey A. Ruhlman and Robert K. Jansen</p><p> </p><p>2. Next Generation Technologies to Determine Plastid Genome Sequences </p><p>Robert J. Henry, Nicole Rice, Mark Edwards and Catherine J. Nock </p><p> </p><p>3. Plastid Gene Transcription: Promoters and RNA Polymerases</p><p>Jennifer Ortelt and Gerhard Link </p><p> </p><p>4. Plastid mRNA Translation</p><p>Masahiro Sugiura </p><p> </p><p>5. Engineering Chloroplasts for High-Level Foreign Protein Expression</p><p>Ralph Bock</p><p>6. Excision of Plastid Marker Genes Using Directly Repeated DNA Sequences</p><p>Elisabeth A. Mudd, Panagiotis Madesis, Elena Martin Avila and Anil Day</p><p> </p><p>7. Fluorescent Labeling and Confocal Microscopic Imaging of Chloroplasts and Non-Green Plastids</p><p>Maureen R. Hanson and Amirali Sattarzadeh</p><p> </p><p> </p><p>Part II: Nicotiana Tabacum the Model Species of Chloroplast Biotechnology</p><p> </p><p>8. Plastid Transformation in Nicotiana tabacum and Nicotiana sylvestris by Biolistic DNA Delivery to Leaves</p><p>Pal Maliga and Tarinee Tungsuchat Huang</p><p> </p><p>9. Nicotiana tabacum – PEG-mediated Plastid Transformation</p><p>Areli Herrera Díaz and Hans-Ulrich Koop</p><p> </p><p>10. Plastid Transformation of Tobacco Suspension Cell Cultures</p><p>Jeffrey M. Staub</p><p> </p><p>11. Tryptophan and Indole Analog Mediated Plastid Transformation</p><p>Pierluigi Barone,  Xing-Hai Zhang and Jack M. Widholm </p><p> </p><p>12. Plastid Marker Gene Excision in Greenhouse-Grown Tobacco by Agrobacterium-Delivered Cre Recombinase</p><p>Tarinee Tungsuchat-Huang and Pal Maliga</p><p> </p><p>13. Determination of the Half-Life of Chloroplast Transcripts in Tobacco Leaves</p><p>Sithichoke Tangphatsornruang and John C. Gray</p><p> </p><p>14. Quantification of Organellar DNA and RNA Using Real-Time PCR</p><p>Andreas Weihe</p><p> </p><p>15. Plastid Transformation for Rubisco Engineering and Protocols for Assessing Expression</p><p>Spencer M. Whitney and Robert E. Sharwood</p><p> </p><p>Part III: Crop Specific Plastid Transformation Protocols</p><p> </p><p>16. Plastid Transformation in Tomato</p><p>Stephanie Ruf and Ralph Bock</p><p> </p><p>17. Stable Plastid Transformation of Petunia</p><p>Elena Martin Avila and Anil Day</p><p> </p><p>18. Plastid Transformation in Potato - Solanum tuberosum</p><p>Vladimir T. Valkov, Daniela Gargano, Nunzia Scotti, and Teodoro Cardi</p><p> </p><p>19. Plastid Transformation in Eggplant </p><p>Kailash C. Bansal and Ajay K. Singh</p> 20. Plastid Transformation in Lettuce (Lactuca sativa L.) by Polyethylene Glycol Treatment of Protoplasts<p>Cilia L.C. Lelivelt, Kees M.P. van Dun, C. Bastiaan de Snoo, Matthew S. McCabe, Bridget V. Hogg, and Jacqueline M. Nugent</p><p> </p><p>21. Plastid Transformation in Lettuce (Lactuca sativa L) by Biolistic DNA Delivery</p><p>Tracey A. Ruhlman</p><p> </p><p>22. Plastid Transformation in Soybean</p><p>Manuel Dubald, Ghislaine Tissot and Bernard Pelissier</p><p> </p><p>23. Plastid Transformation in Cabbage (Brassica oleracea L. var. capitata L.) by the Biolistic Process</p><p>Menq-Jiau Tseng, Ming-Te Yang, Wan-Ru Chu, and Cheng-Wei Liu</p><p> </p><p>24. Plastid Transformation in Sugar Beet – Beta vulgaris</p><p>Francesca De Marchis and Michele Bellucci</p><p> </p><p>25. Integration and Expression of gfp in the Plastid of Medicago sativa L.</p><p>Shaochen Xing, Zhengyi Wei, Yunpeng Wang, Yanzhi Liu, Chunjing Lin</p><p> </p><p>Part IV: Plastid Transformation in Algae</p><p>26. Rapid Screening for the Robust Expression of Recombinant Proteins in Algal Plastids</p><p>Daniel Barrera, Javier Gimpel and Stephen Mayfield</p><p> </p><p>27. A Simple, Low Cost Method for Chloroplast Transformation of the Green Alga Chlamydomonas reinhardtii</p><p>Chloe Economou, Thanyanan Wannathong, Joanna Szaub and Saul Purton</p><p> </p><p>28. Tools for Regulated Gene Expression in the Chloroplast of Chlamydomonas</p><p>Jean-David Rochaix, Raymond Surzycki and Silvia Ramundo </p><p> </p><p>Part V : Plastid Transformation in Bryophyte</p><p>29. Plastid Transformation in Physcomitrella patens</p><p>Mamoru Sugita</p><p> </p><p>30. Plastid Transformation of Sporelings and Suspension-cultured Cells from the Liverwort Marchantia polymorpha L.</p><p>Shota Chiyoda, Katsuyuki T. Yamato, and Takayuki Kohchi</p><p> </p>