MicroRNAs as Tools in Biopharmaceutical Production
Samenvatting
Focused manuscript on the potential use/role of miRNAs in bioprocessing, specifically the production of complex proteins in mammalian cells. With that in mind I propose a draft list of topics/chapters along the following lines: Intro on CHO/bioprocessing/engineering challenges to set scene, Genomic organization, biogenesis and mode of action, Identifying miRNA targets: Computational prediction, transcriptomics, proteomices, UTR analysis, etc., miRNA expression in Chinese Hamster Ovary cells, miRNAs as engineering targets: pathway manipulation to impact bioprocess phenotypes, miRNAs as biomarkers, Detection methods: Northern, PCR, hybridization arrays, Next Gen Seq, Manipulation of expression in cultured cells: Transient/stable disregulation, Knockout.
Specificaties
Inhoudsopgave
1 A general introduction to microRNAs, their investigation and exploitation in CHO cell lines
2 Biogenesis of Mammalian miRNA
3 Target prediction algorithms and bioinformatics resources for miRNA studies
4 The CHO miRNA transcriptome
5 MicroRNAs as engineering targets - pathway manipulation to impact bioprocess phenotypes
6 MicroRNAs for Enhancement of CHO Cell Proliferation and Stability Insights from Neuroblastoma Studies
7 MicroRNA Detection Methods for Mammalian Cell Lines and Their Applications in Therapeutic Protein Production
8 Manipulating Levels of Specific microRNAs in Mammalian Cells
Index.
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