Handbook of Vascular Biology Techniques

<p>Preface</p><p>Section 1 In vitro techniques</p><p>Chapter 1: In vitro angiogenesis assay - endothelial migration, proliferation, and tube formation</p><p>Chapter 2: Endothelial cell tube formation on basement membrane to study cancer neoangiogenesis</p><p>Chapter 3: Induction of hypoxia in vascular endothelial cell culture</p><p>Chapter 4: Evaluating In Vitro Angiogenesis using Live Cell Imaging</p><p>Chapter 5: Isolation of Endothelial Progenitor Cells (EPCs)</p><p>Chapter 6: Culture and Maintenance of Human Embryonic Stem Cells: A Potential Source for Vasculargenesis</p><p>Chapter 7: Assessment of vascular function and contractility, ex vivo.</p><p>Chapter 8: Measurement of Aβ uptake by cerebrovascular smooth muscle cells</p><p>Chapter 9: Measurement of Intracellular Ca2+ in Human Endothelial cells</p><p> </p><p>Section 2: In vivo and ex vivo manipulations</p><p>Chapter 10: Evaluation of angiogenesis and arteriogenesis in a mouse model of prolonged cerebral hypoperfusion</p><p>Chapter 11: Sponge implant model of inflammatory angiogenesis</p><p>Chapter 12: The chick embryo chorioallantoic membrane assay</p><p>Chapter 13: Dorsal air sac assay</p><p>Chapter 14: Scanning Electron Microscopy of Blood Vascular Corrosion Casts (SEM on VCC) in mammals</p><p>Chapter 15: Hypoxia-induced retinal angiogenesis in adult zebrafish</p><p>Chapter 16: Angiogenesis in regenerating adult zebrafish tail fin</p><p>Chapter 17: Methods for studying developmental angiogenesis in zebrafish</p><p>Chapter 18: Isolation and Expansion of Brain Microvascular Endothelial Cells</p><p> </p><p>Section 3: Imaging and histological analysis</p><p>Chapter 19: Adipose angiogenesis</p><p>Chapter 20: Assessing tumor angiogenesis in histological samples</p><p>Chapter 21: Whole-mount immunostaining methods to study the blood and lymphatic vasculature in the  embryonic mouse skin and adult mouse cornea.</p><p>Chapter 22: Computed Tomography Angiography: Fundamental techniques and data interpretation</p><p>Chapter 23: Magnetic Resonance Angiography: Fundamental Techniques and Data Interpretation</p><p>Chapter 24: Single-Photon Emission Tomography of the brain in vascular pathology</p><p> </p><p>Section 4: Miscellaneous novel techniques in vascular biology</p><p>Chapter 25: Enhancing Endothelialisation of Artificial/Engineered Blood Vessels Using Structural Cues</p><p>Chapter 26: Preparation of liposomes with dual fluorophores to follow real-time content release in vivo </p><p>Chapter 27: Vascular Flow Modelling using Computational Fluid Dynamics</p><p>Chapter 28: Reverse Transcription Real-Time PCR Protocol for gene expression analyses</p><p>Chapter 29: Oscillations, Feedback and Bifurcations in Mathematical Models of Angiogenesis and Haematopoiesis</p><p>Chapter 30: Genomic microarray analysis</p><p>Chapter 31: Selection of appropriate housekeeping genes for quality control</p><p>Chapter 32: Endothelial transcriptomic analysis</p><p>Chapter 33: Protocol for Multiplex Amplicon Sequencing using Barcoded Primers</p><p>Chapter 34: Flow cytometry Enumeration of Hematopoietic &amp; Progenitor Stem Cells:  Identification and Quantification</p><p>Chapter 35: A Scheme for the Development and Validation of Enzyme Linked Immunosorbent Assays (ELISA) for Measurement of Angiogenic Biomarkers in Human Blood</p><p>Chapter 36: Analysis of Phosphorylated Protein Kinases in Endothelial cells by Flow Cytometry</p>