2D and 3D Cellular Screening Models and AI Guided Analysis

1. Preface<br>Guido Kroemer and Oliver Kepp<br>2. High throughput CellTiter-glo viability assessment<br>Dana Alawieh and Aline Renneville<br>3. Assessing viability of cancer cell spheroids in 3D drug screening<br>Oliver Kepp and Marion Leduc<br>4. 3D killing assay of cancer spheroids by cytotoxic T lymphocytes in anchored microfluidic droplets<br>Charles Baroud<br>5. Use of micropatterning for 3D cell culture<br>Emna Ouni and Kristine Schauer<br>6. Use of microfluidic chip devices for assessing the effect of radiation on 3D cell culture models<br>Philip Wong, Rodin Chermat, Elena Refet-Mollof, Maryam Ziaee, Julie Lafontaine and Thomas Gervais<br>7. A 3D model for assessing regulated cell death modalities<br>Dmitri Krysko and Robin Demuynck<br>8. YOLO detection model for Cell Viability assessment<br>Felix Peyre<br>9. Bioinformatics and AI/ML approaches using multi-omics data to accelerate diagnosis and delivery of precision care<br>Zeeshan Ahmed, Kritika Singh, Sarah Usman, Saman Zeeshan, Naveena Yanamala, Vikram Bhise, Mark Nichols, Sabahat Bokhari and Partho Sengupta<br>10. Automated image analysis and machine learning for the assessment of mitochondrial membrane potential<br>Marta Giacomello, Camilla Bean, Federica Dal bello, Emad Norouzi Esfahani, Alessio Gianelle, Saradha Ramesh, Luca Persano, Elena Rampazzo and Caterina Vianello<br>11. Assessing cancer cell heterogeneity in patient-derived tumoroids<br>Flora Doffe<br>12. Assessment of lysosomal drug sequestration and release using fluorescence microscopy<br>Karla Alvarez, Mojgan Djavaheri-Mergny, Guido Kroemer, Lucille Ferret, Samy Dehissi and Jean Guillon<br>13. Assessment of T cell toxicity in ex-vivo models of cancer<br>Emmanuel intégrative des tumeurs et immunothérapie Donnadieu and Alice Machado<br>14. Three-dimensional Organoid Screening using Leica Thunder Microscope<br>Manoliu Tudor<br>15. Using vascularized organoids-on-chip for assessing systemic drug delivery<br>Xavier Gidrol, Emily Tubbs, Fabrice P Navarro and Yves Fouillet<br>16. Quantitative assessment of nanoparticle uptake and trafficking in 3D cell culture models<br>Meritxell B. Cutrona<br>17. Fluorescence microscopic quantitation of lysosomal cholesterol accumulation induced by cationic amphiphilic drugs<br>Mojgan Djavaheri-Mergny, Karla Alvarez, Samy Dehissi, Lucille Ferret and Guido Kroemer