Laboratory Exercises in Microbiology

PART ONE<br/>Microscopic Techniques<br/>1. Bright-Field Light Microscopy and Microscopic Measurement of Organisms<br/>2. The Hanging Drop Slide and Bacterial Motility<br/>3. Dark-Field Light Microscopy<br/>4. Phase-Contrast Microscopy<br/>PART TWO<br/>Bacterial Cell Biology<br/>5. Aseptic Technique<br/>6. Negative Staining<br/>7. Simple Staining<br/>8. Gram Stain<br/>9. Acid-Fast Staining Procedure<br/>10. Endospore Staining<br/>11. Capsule Staining<br/>12. Flagella Staining<br/>PART THREE<br/>Basic Culture Techniques<br/>13. Preparation of Microbiology Media and Equipment<br/>14. The Spread-Plate Technique<br/>15. The Streak-Plate Technique<br/>16. The Pour-Plate Technique<br/>17. Cultivation of Anaerobic Bacteria&nbsp;<br/>18. Determination of Bacterial Numbers<br/>PART FOUR<br/>Microbial Biochemistry<br/>19. Carbohydrates I: Fermentation and β-Galactosidase Activity<br/>20. Carbohydrates II: Triple Sugar Iron Agar Test<br/>21. Carbohydrates III: Starch Hydrolysis<br/>22. Lipid Hydrolysis<br/>23. Proteins I: The IMViC Tests<br/>24. Proteins II: Gelatin and Casein Hydrolysis<br/>25. Proteins III: Catalase Activity<br/>26. Proteins IV: Oxidase Test<br/>27. Proteins V: Urease Activity<br/>28. Proteins VI: Lysine and Ornithine Decarboxylase Tests<br/>29. Proteins VII: Phenylalanine Deamination<br/>30. Proteins VIII: Dissimilatory Nitrate Reduction<br/>PART FIVE<br/>Environmental Factors Affecting Growth of Microorganisms<br/>31. Temperature<br/>32. pH<br/>33. Osmotic Pressure<br/>34. The Effects of Chemical Agents on Bacteria I: Disinfectants and Other Antimicrobial Products<br/>35. The Effects of Chemical Agents on Bacteria II: Antibiotics<br/>36. Bacterial Growth Curve<br/>PART SIX<br/>Environmental and Food Microbiolog<br/>37. Monitoring Water for Coliforms<br/>38. Enumeration of Soil Microorganisms<br/>39. Winogradsky Columns<br/>40. Bioluminescence<br/>41. Plate Counts and Quality Assessment of Milk<br/>PART SEVEN<br/>Medical Microbiology<br/>42. Staphylococci<br/>43. Pneumococcus<br/>44. Streptococci<br/>45. Neisseriae<br/>46. Normal Human Flora<br/>PART EIGHT<br/>Eukaryotic Microbiology<br/>47. Fungi I: Yeasts (Ascomycota)<br/>48. Fungi II: Zygomycota (Rhizopus), Ascomycota (Penicillium), and Basidiomycota (Agaricus)<br/>