Matthew C. Gage,
Inés Pineda-Torra
Springer New York
e druk, 2019
9781493991297
Lipid-Activated Nuclear Receptors
Methods and Protocols
Specificaties
Gebonden, blz.
|
Engels
Springer New York |
e druk, 2019
ISBN13: 9781493991297
Rubricering
Juridisch
:
Onderdeel van serie
Methods in Molecular Biology
Verwachte levertijd ongeveer 9 werkdagen
Samenvatting
This book covers a wide range of state-of-the-art methodologies and detailed protocols currently used to study the actions that lipid-activated nuclear receptors and their co-regulators have in tissues and immune cell types considered classic metabolic “powerhouses”. This includes the liver, adipose tissue, and monocytes/macrophages present in these and other metabolic tissues. While the main focus is on the oxysterol receptor or Liver X Receptor (LXR), the majority of the methods described can be easily applied to multiple nuclear receptors, as well as to other tissues or cell types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Lipid-Activated Nuclear Receptors: Methods and Protocols serves as an ideal guide for researchers pursuing the vital study of nuclear receptor biology and beyond.
Specificaties
ISBN13:9781493991297
Taal:Engels
Bindwijze:gebonden
Uitgever:Springer New York
Inhoudsopgave
<p>1. Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS</p>
<p> Lilia Magomedova and Carolyn L. Cummins</p>
<p> </p>
<p>2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands</p>
<p> Samantha A. Hutchinson and James L. Thorne</p>
<p> </p>
<p>3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages</p>
<p>Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, and Fawaz Alzaid</p>
<p> </p>
<p>4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages</p>
<p> Emelie Barreby, André Sulen, and Myriam Aouadi</p>
<p> </p>
<p>5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity</p>
Laura Alonso-Herranz, Jesús Porcuna, and Mercedes Ricote<p></p>
<p> </p>
<p>6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells</p>
<p> Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo</p>
<p> </p>
<p>7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages</p>
<p> David A. Rollins and Inez Rogatsky</p>
<p> </p>
<p>8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics</p>
<p> Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, and Antonio Castrillo</p>
<p> </p>
<p>9. Quantifying Cellular Cholesterol Efflux</p>
<p> Sabrina Robichaud and Mireille Ouimet</p>
<p> </p>
<p>10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection</p>
<p> Estibaliz Glaría, Jonathan Matalonga, and Annabel F. Valledor</p>
<p> </p>
<p>11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency</p>
<p> Matthew C. Gage</p>
<p> </p>
<p>12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution</p>
<p> Ada Weinstock and Edward A. Fisher</p>
<p> </p>
<p>13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis</p>
<p> Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter</p>
<p> </p>
<p>14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver</p>
<p> Natalia Becares and Inés Pineda-Torra</p>
<p> </p>
<p>15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo</p>
<p> Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Hélène Duez, and Benoit Pourcet</p>
<p> </p>
<p>16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry</p>
<p> Kirsty E. Waddington, Inés Pineda-Torra, and Elizabeth C. Jury</p>
<p> Lilia Magomedova and Carolyn L. Cummins</p>
<p> </p>
<p>2. A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands</p>
<p> Samantha A. Hutchinson and James L. Thorne</p>
<p> </p>
<p>3. Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages</p>
<p>Jean-Baptiste Julla, Raphaelle Ballaire, Tina Ejlalmanesh, Jean-François Gautier, Nicolas Venteclef, and Fawaz Alzaid</p>
<p> </p>
<p>4. Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages</p>
<p> Emelie Barreby, André Sulen, and Myriam Aouadi</p>
<p> </p>
<p>5. Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity</p>
Laura Alonso-Herranz, Jesús Porcuna, and Mercedes Ricote<p></p>
<p> </p>
<p>6. Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells</p>
<p> Ana Ramón-Vázquez, Juan Vladimir de la Rosa, Carlos Tabraue, and Antonio Castrillo</p>
<p> </p>
<p>7. Dual Crosslinking Chromatin-Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages</p>
<p> David A. Rollins and Inez Rogatsky</p>
<p> </p>
<p>8. Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics</p>
<p> Juan Vladimir de la Rosa, Ana Ramón-Vázquez, Carlos Tabraue, and Antonio Castrillo</p>
<p> </p>
<p>9. Quantifying Cellular Cholesterol Efflux</p>
<p> Sabrina Robichaud and Mireille Ouimet</p>
<p> </p>
<p>10. Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection</p>
<p> Estibaliz Glaría, Jonathan Matalonga, and Annabel F. Valledor</p>
<p> </p>
<p>11. Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency</p>
<p> Matthew C. Gage</p>
<p> </p>
<p>12. Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution</p>
<p> Ada Weinstock and Edward A. Fisher</p>
<p> </p>
<p>13. Preparation of Frozen Liver Tissues for Integrated Omics Analysis</p>
<p> Ning Liang, Rongrong Fan, Saioa Goni, and Eckardt Treuter</p>
<p> </p>
<p>14. Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver</p>
<p> Natalia Becares and Inés Pineda-Torra</p>
<p> </p>
<p>15. The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo</p>
<p> Yasmine Sebti, Lise Ferri, Mathilde Zecchin, Justine Beauchamp, Denis Mogilenko, Bart Staels, Hélène Duez, and Benoit Pourcet</p>
<p> </p>
<p>16. Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry</p>
<p> Kirsty E. Waddington, Inés Pineda-Torra, and Elizabeth C. Jury</p>
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