Computer Optimized Microscopy

<div>Main Steps in Image Processing and Quantification: The Analysis Workflow.-&nbsp;Open Source Tools for Biological Image Analysis.-&nbsp;Proximity Ligation Assay Image Analysis Protocol: Addressing Receptor-Receptor Interactions.-&nbsp;Introduction to ImageJ Macro Language in a Particle Counting Analysis: Automation Matters.-&nbsp;Automated Macro Approach to Quantify Synapse Density in 2D Confocal Images from Fixed Immunolabeled Neural Tissue Sections.-&nbsp;Automated Quantitative Analysis of Mitochondrial Morphology.-&nbsp;Structure and Fluorescence Intensity Measurements in Biofilms.-&nbsp;3D+Time Imaging and Image Reconstruction of Pectoral Fin during Zebrafish Embryogenesis.-&nbsp;Automated Macro Approach to Remove Vitelline Membrane Autofluorescence in Drosophila Embryo 4D Movies.-&nbsp;Which Elements to Build Co-Localization Workflows? From Metrology to Analysis.-&nbsp;A Triple Colocalization Approach to Assess Traffic Patterns and their Modulation.-&nbsp;Photobleaching and Sensitized Emission Based Methods for the Detection of Förster Resonance Energy Transfer.-&nbsp;In Vivo Quantification of Intramolecular FRET using RacFRET Biosensors Manel.-&nbsp;Cell Proliferation High-Content Screening on Adherent Cell Cultures.-&nbsp;HCS Methodology for Helping in Lab Scale Image-Based Assays.-&nbsp;Filopodia Quantification using FiloQuant.-&nbsp;Coincidence Analysis of Molecular Dynamics by Raster Image Correlation.-&nbsp;3D Tracking of Migrating Cells from Live Microscopy Time-Lapses.-&nbsp;A Cell Segmentation/Tracking Tool Based on Machine Learning.-&nbsp;2D+Time Object Tracking using Fiji and ilastik.-&nbsp;Machine Learning: Advanced Image Segmentation using ilastik.</div>