Plant and Food Carotenoids

<p>Pathways for Carotenoid Biosynthesis, Degradation, and Storage.-&nbsp;A Simple in vitro Assay to Measure the Activity of Geranylgeranyl Diphosphate Synthase and Other Short-chain Prenyltransferases.-&nbsp;Expression, Purification, and Enzyme Activity Assay of Phytoene Synthase in vitro.-&nbsp;Improved Expression and Purification of the Carotenoid Biosynthetic Enzyme Z-ISO.-&nbsp;Determination of In Vitro and In Vivo Activities of Plant Carotenoid Cleavage Oxygenases.-&nbsp;Expression and Characterization of Mammalian Carotenoid Cleavage Dioxygenases.-&nbsp;Transportomics for the Characterization of Plant Apocarotenoid Transmembrane Transporters.-<strong>&nbsp;</strong>Applications of Visible Spectroscopy and Colour Measurements in the Assessments of Carotenoid Levels in Foods.-&nbsp;A Routine Method for the Extraction and HPLC-DAD Profiling of Major Plant and Food Carotenoids.-&nbsp;Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management.-&nbsp;cis/trans Carotenoid Extraction, Purification, Detection, Quantification, and Profiling in Plant Tissues.-&nbsp;Determination of Plant Volatile Apocarotenoids.-&nbsp;A Method for Extraction and LC-MS Based Identification of Carotenoid-Derived Dialdehydes in Plants.-&nbsp;Fractionation of Tomato Fruit Chromoplasts.-&nbsp;Quantification of Strigolactones.-&nbsp;Carotenoid and Apocarotenoid Analysis by SFE-SFC-QqQ/MS.-&nbsp;Elucidating Carotenoid Biosynthetic Enzyme Localization and Interactions Using Fluorescent Microscopy.-&nbsp;Visualization of Carotenoid-storage Structures in Fruits by Transmission Electron Microscopy.-&nbsp;&nbsp;Light Microscopy and Raman Imaging of Carotenoids in Plant Cells in situ and in Released Carotene Crystals.-&nbsp;Carotenoid Isotopologue Profiling in ¹³C-labeled Leaf Extracts by LC-MS and LC-FTICR-MS.-&nbsp;Quantification of Carotenoid Pathway Flux in Green and Non-green Systems.-&nbsp;Histochemical and Molecular Quantification of Arbuscular Mycorrhiza Symbiosis.-&nbsp;Use of Potyvirus Vectors to Produce Carotenoids in Plants.-&nbsp;Agrobacterium tumefaciens Mediated Stable Transformation of Daucus carota.-&nbsp;CRISPR-Cas-Mediated Gene Knockout in Tomato.-&nbsp;Modulation of Activity of a Carotenoid Pathway Through the use of the TET-on Regulatory System: Application in the Fungus Fusarium fujikuroi.-&nbsp;Intervention Studies in Humans.-&nbsp;In vitro Digestion for Control and Monitoring of Food Effects In Relation To Micellarization Index of Carotenoids.-&nbsp;Evaluation of Carotenoids Protection against Oxidative Stress in the Animal Model Caenorhabditis elegans.-&nbsp;Mouse Models to Study Antiobesogenic Effects of Carotenoids.<br></p>

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