Clinical Applications of PCR

Specificaties
Gebonden, 200 blz. | Engels
Humana Press | 2006e druk, 2006
ISBN13: 9781588293480
Rubricering
Juridisch :
Humana Press 2006e druk, 2006 9781588293480
Onderdeel van serie Methods in Molecular Biology
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Samenvatting

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Specificaties

ISBN13:9781588293480
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:200
Uitgever:Humana Press
Druk:2006

Inhoudsopgave

to the Polymerase Chain Reaction.- Setting Up a Polymerase Chain Reaction Laboratory.- Real-Time Polymerase Chain Reaction and Melting Curve Analysis.- Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses.- In-Cell Polymerase Chain Reaction.- Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry.- Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography.- Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies.- Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma.- Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA.- Molecular Analysis of Circulating RNA in Plasma.- Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction.- Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis.- Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction.- Molecular Diagnosis of Severe Acute Respiratory Syndrome.- Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus.

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        Clinical Applications of PCR