Peptide Characterization and Application Protocols

Specificaties
Gebonden, 342 blz. | Engels
Humana Press | 2007e druk, 2007
ISBN13: 9781588295507
Rubricering
Juridisch :
Humana Press 2007e druk, 2007 9781588295507
Onderdeel van serie Methods in Molecular Biology
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Samenvatting

Peptide Characterization and Application Protocols is dedicated entirely to the characterization of peptides and their applications for the study of biochemical systems and the contributing authors are all leaders in the field of peptide research. Part I, Characterization, presents the most recent advances in select analytical techniques, including high-performance liquid chromatography (HPLC) for purification and evaluation of peptides and mass spectrometry (MS) for the analysis of standard and modified peptides. Innovative methods of synthesis and characterization of membrane peptides are also presented. Part II, Application, presents a variety of specific applications for synthetic peptides. The collected protocols demonstrate the systematic manner in which peptides can be constructed, analyzed, and applied to attack contemporary problems in biochemistry, and this book will be an indispensable aid in the pursuit of new directions in peptide research.

Specificaties

ISBN13:9781588295507
Taal:Engels
Bindwijze:gebonden
Aantal pagina's:342
Uitgever:Humana Press
Druk:2007

Inhoudsopgave

Characterization.- HPLC Analysis and Purification of Peptides.- Identification of Proteins Based on MS/MS Spectra and Location of Posttranslational Modifications.- Determination of Phosphorylated and O-Glycosylated Sites by Chemical Targeting (CTID) at Ambient Temperature.- Synthesis, Biosynthesis, and Characterization of Transmembrane Domains of a G Protein—Coupled Receptor.- Applications.- Application of Topologically Constrained Mini-Proteins as Ligands, Substrates, and Inhibitors.- Proteolytic Profiling of the Extracellular Matrix Degradome.- ?-Amyloid Protein Aggregation.- Radiometal-Labeled Somatostatin Analogs for Applications in Cancer Imaging and Therapy.- Cell-Penetrating Proline-Rich Peptidomimetics.- Peptide-Mediated Targeting of Liposomes to Tumor Cells.- Peptide-Mediated Delivery of Nucleic Acids into Mammalian Cells.- Broth Microdilution Antibacterial Assay of Peptides.- Depsipeptide Synthesis.

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        Peptide Characterization and Application Protocols