Lipid Rafts

Overview of Membrane Rafts.- Analysis of Raft Affinity of Membrane Proteins by Detergent-Insolubility.- Nondetergent Isolation of Rafts.- Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo.- Using Monomolecular Films to Characterize Lipid Lateral Interactions.- Electro-Formation and Fluorescence Microscopy of Giant Vesicles With Coexisting Liquid Phases.- Fluorescence Correlation Spectroscopy.- Multiphoton Laser-Scanning Microscopy and Spatial Analysis of Dehydroergosterol Distributions on Plasma Membrane of Living Cells.- NMR Detection of Lipid Domains.- Lateral Diffusion Coefficients of Raft Lipids From Pulsed Field Gradient NMR.- Saturation-Recovery Electron Paramagnetic Resonance Discrimination by Oxygen Transport (DOT) Method for Characterizing Membrane Domains.- Plasmon-Waveguide Resonance Spectroscopy Studies of Lateral Segregation in Solid-Supported Proteolipid Bilayers.- Fluorescence Recovery After Photobleaching Studies of Lipid Rafts.- Single-Molecule Tracking.- X-Ray Diffraction to Determine the Thickness of Raft and Nonraft Bilayers.- Small-Angle Neutron Scattering to Detect Rafts and Lipid Domains.- Exploring Membrane Domains Using Native Membrane Sheets and Transmission Electron Microscopy.- Atomic Force Microscopy of Lipid Domains in Supported Model Membranes.- Atomistic and Coarse-Grained Computer Simulations of Raft-Like Lipid Mixtures.- A Microscopic Model Calculation of the Phase Diagram of Ternary Mixtures of Cholesterol and Saturated and Unsaturated Phospholipids.