Antibiotic Resistance Protocols
Second Edition
Samenvatting
Since the publication of the popular first edition, genomic methods have become more accessible, allowing antibiotic researchers to probe not only the sequence of antibiotic resistance determinants but the mechanism whereby they are expressed and regulated. That, in concert with array technology and an understanding of the importance of biofilms, has greatly expanded antibiotic resistance knowledge. In order to reflect the growing field, Antibiotic Resistance Protocols, Second Edition fully updates and builds upon its first edition with contributions from leading researchers. Beginning with chapters on epidemiology and population genetics, the book continues with sections covering genomics and gene expressions, fitness mutation and physiology, and the detection of resistance. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Antibiotic Resistance Protocols, Second Edition brings together examples of a diverse range of modern techniques applied in antibiotic research in order to best aid scientists in planning their own future research.
Specificaties
Inhoudsopgave
1. The RM Test for Determining Methicillin-Resistant Staphylococcus aureus (MRSA) Lineages
Jodi A. Lindsay and Julia M.-L. Sung
2. Unravelling the Ecology of Antibiotic Resistant Bacteria in the Nasopharynx
Bambos M. Charalambous and Ndeky M. Oriyo
Part II: Genomics and Gene Expression
3. Methods to Determine Antibiotic Resistance Gene Silencing
Virve I. Enne and Peter M. Bennett
4. Studying Gene Induction of Glycopeptide Resistance Using Gene Swapping
Hee-Jeon Hong
5. Real Time PCR Methods to Study Expression of Genes Related to Hypermutability
Denise O’Sullivan
6. Use of DNA Arrays to Study Transcriptional Responses to Antimycobacterial Compounds
Simon. J. Waddell and Philip D. Butcher
Part III: Fitness Mutation and Physiology
7. Assays of Sensitivity of Antibiotic-Resistant Bacteria to Hydrogen Peroxide and Measurement of Catalase Activity
Mirjana Macvanin and Diarmaid Hughes
8. Measurements of Heme Levels and Respiration Rate in Salmonella enterica serovar Typhimurium
Diarmaid Hughes and Mirjana Macvanin
9. Methods to Determine Fitness in Bacteria
Cassie F. Pope, Timothy D. McHugh, and Stephen H. Gillespie
10. Application of Continuous Culture for Measuring the Effect of Environmental Stress on Mutation Frequency in Mycobacterium tuberculosis
Joanna Bacon, Kim A. Hatch, and Jon Allnutt
Part IV: Detection of Resistance
11. Identification of Efflux-Mediated Multi-Drug Resistance in Bacterial Clinical Isolates by Two Simple Methods
Marta Martins, Isabel Couto, Miguel Viveiros, and Leonard Amaral
12. Evaluation of Efflux Activity of Bacteria by a Semi-Automated Fluorometric System
Miguel Viveiros, LilianaRodrigues, Marta Martins, Isabel Couto, Gabriella Spengler, Ana Martins, and Leonard Amaral
13. Measuring the Activity of Active Efflux in Gram-Negative Bacteria
Mark A. Webber and Nick G. Coldham
14. Rapid Characterization of b -Lactamases by Multiplex PCR
Neil Woodford
15. Rapid Methods for Testing Inhibitors of Mycobacterial Growth
Dimitrios Evangelopoulos and Sanjib Bhakta
16. Use of Biofilm Model Systems to Study Antimicrobial Susceptibility
Jonathan Pratten and Derren Ready
17. Rapid Analysis of Resistant Mutant Genotypes Using Pyrosequencing®
Catherine Arnold
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