HPLC Methods for Clinical Pharmaceutical Analysis – A User′s Guide
A User′s Guide
Samenvatting
Filling a gap in the literature for a hands–on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of
experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walkthrough case studies. Based on a course taught by the author, the first part of the book intuitively explains all steps of routine bioanalysis and explains how to set up a robust, inexpensive and effi cient method for a given substance. In the second part he includes 20 worked example cases that highlight common challenges and how to overcome them.
With its appendix containing tried–and–tested analytical methods for 100 clinically relevant substances from the author`s own laboratory,
complete with spectral and MS data as well as literature references and basic pharmacokinetic information, this is a life–long companion for
everyone working in clinical, pharmaceutical and biochemical analysis.
Comments to the German book:
"The book comes to life through its examples, showing not only what did work in the author′s laboratory, but also what didn′t."
ChemieReport
"Indispensable for novices, while even old hands will be able to expand their knowledge. A collection of analytical data for ca. 100 substances completes the book′s offering, leaving almost nothing to be desired."
pharmind
Specificaties
Inhoudsopgave
<br />
<br /> INTRODUCTION
<br /> First Question: Determination of Ibuprofen in Plasma
<br /> Second Question: Determination of Tryptophan in Urine
<br /> Third Question: Determination of Paclitaxel in Tissue
<br />
<br /> PLANNING OF ANALYSES
<br /> Introduction
<br /> Limit of Detection (LOD) and Determination (LLOQ)
<br /> Detectors
<br /> Structure of the Analyte
<br /> Solubility of an Analyte
<br /> Selection of the Detector
<br />
<br /> SAMPLE PREPARATION
<br /> Dilution
<br /> Protein Precipitation, Overview
<br /> Extraction
<br />
<br /> HPLC SEPARATION
<br /> HPLC Pumps
<br /> Degassers
<br /> Injector
<br /> HPLC Columns
<br />
<br /> DETECTION
<br /> Detection in the Pharmaceutical/Bioanalytical Area
<br /> Detection in the "Clinical Area" (Therapy Control/Compliance)
<br />
<br /> CHEMICAL DERIVATISATION FOR DETECTION ENHANCEMENT
<br />
<br /> VALIDATION CONCEPTS
<br /> Introduction
<br /> Realisation of the FDA Guideline
<br />
<br /> PRACTICAL HINTS CONCERNING STABILITY, DESTRUCTION AND DEGRADATION PRODUCTS
<br />
<br /> METABOLITES
<br />
<br /> INTERNAL STANDARDS
<br />
<br /> CASE STUDIES WITH INTENSIVE DISCUSSION FOR EACH SUBSTANCE
<br /> Acetylcarnitine in Plasma
<br /> Acetylcysteine in Plasma
<br /> Acyclovir in Plasma and Urine
<br /> Caffeine in Plasma
<br /> Diazepam in Plasma
<br /> Diclofenac in Plasma
<br /> Dihydralazine in Plasma
<br /> Duramycin (Moli1901) in Plasma
<br /> Fluticasone Propionate in Plasma
<br /> Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine
<br /> Ibuprofen in Plasma (also Enantiomeric Separation)
<br /> Minocycline in plasma
<br /> Norfloxacine in Plasma and Urine
<br /> Paclitaxel in Plasma, Urine, and Tissue
<br /> Paracetamol (Acetaminophen) in Plasma
<br /> Pimelic Acid in Plasma and Urine
<br /> 8–Prenylnaringenin in Plasma and in Different Types of Tissues
<br /> Silibinin in Plasma
<br /> Valnemulin in Plasma, Different Tissue Types and Animal Feed
<br /> Vitamin B1 (Total Thiamine) in Plasma
<br />
<br /> APPENDIX
<br /> Short Description of Determination for about 100 Substances
<br /> Substances listed in the Appendix
<br /> A Short Explanation of Tables Presented in the Appendix
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